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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in lots of tumors, which leads to chromosome instability, and thus may perhaps add to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and plays a crucial position in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have been described. Two further splice variants have been described but specific nucleotide sequence details is not really yet out there.
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Blocking peptides are peptides that bind particularly into the focus on antibody and block antibody binding. These peptide normally incorporates the epitope identified via the antibody.
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This manual outlines additional feasible optimization of cellular and immunoassay parameters to ensure the absolute best benefits are...
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This guideline outlines further more possible optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...
Antibodies bound to the blocking peptide not bind towards the epitope to the target protein. By comparing the staining in the blocked antibody versus the antibody by itself, you can see which staining is distinct.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance through the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful check here gizmo for measuring the modulation of receptor activation by the two antibodies...
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